Cell Surface Engineering with Synthetic and Natural LBL Nanoshells

Vladimir Tsukruk
Georgia Institute of Technology
Friday, March 1, 2013
NCSU, Room 1011, Engineering Building 1 | 11:00am

Note:  This seminar will be given both at Duke on 2/28/13 (3pm, Schiciano B, Fitzpatrick Center) and at NCSU on 3/1/13 (11am, Room 1011, EB1).

We discuss recent results on designing highly permeable, gel, and robust nanoscale shells as prospective delivery vehicles and conformal cell protection. Ultrathin shells from synthetic and natural , materials are assembled in order to conduct surface modification and protection of model microparticles, cells and cell assemblies . Shells designed here were formed through a layer-by-layer (LbL) assembly based upon hydrogen-bonding, ion pairing, and hydrophobic-hydrophobic interactions. These LbL shells can be reinforced via physical or covalent crosslinking by inclusion of nanoparticles, inducing local crystallization, or adding crosslinking agents and mechanical properties can be tested with surface force spectroscopy. These soft shells are further exploited to control and tune the fractal morphology, permeability, biodegradability, and compliance of the LbL shells. , , We demonstrated that the pH-responsive outer coatings support high viability of the cells with minor effect of growth and dividing of cells especially if a cationic component is screened or completely removed. The important cell function to express green fluorescent protein was demonstrated to be preserved for a long time and can be controlled by external stimuli. In this presentation, we will discuss the sophisticated control in cell viability, the preservation of cell functioning, microcapsule shape preservation under variable pressure, pH-responsive behavior, and significance of core-shell morphologies and shapes with tunable loading-unloading properties, enhanced robustness, and guided assembly.

Vladimir V. Tsukruk received his MS degree in physics from the National University of Ukraine, and his PhD and DSc in chemistry and polymer science from the National Academy of Sciences of Ukraine. He carried out his post-doc research at the U. Marburg and U. Akron. He is currently a Professor at the School of Materials Science and Engineering, Georgia Institute of Technology and a co-Director of Air Force BIONIC Center of Excellence. He serves on the editorial advisory boards of six professional journals, has co-authored around 350 refereed articles in archival journals, as well as five books and has organized ten professional symposia, and trained about 60 students and post-docs. His research in the fields of surfaces & interfaces, molecular assembly, nano- and bioinspired materials has been recognized by the Humboldt Research Award (2009) and the NSF Special Creativity Award (2006), among others and he was elected an APS Fellow in 2010 and an MRS Fellow in 2011. His recent book Scanning Probe Microscopy of Soft Matter: Fundamentals and Practices, Wiley, 2012 is a comprehensive textbook on SPM applications of synthetic and biological materials.