Clinical diagnostic assays for detecting biomarkers can potentially help in the identification and epidemiological tracking of many diseases, as well as in their treatment monitoring. Point-of-care immunoassays such as lateral flow tests are cost-effective analytical tests, which can be performed in low-resource settings with untrained users to provide more immediate diagnosis. However, the major drawback is poor sensitivity. To address the need, we have developed a family of stimuli-responsive reagents that can enable easy-to-use rapid diagnostic assays to achieve ELISA level sensitivity. The new reagent systems can isolate full or half-sandwich immunocomplexes from human plasma and directly interface with different diagnostic devices such as microfluidic cards and non-instrumented flow-through devices. Additionally, the rapid assay system is scalable to larger specimen volumes (≥ 10x), which provides opportunities to concentrate dilute biomarkers, thus improving detection ranges and expanding diagnostic options in point-of-care immunoassays. In this presentation, I will describe how the reagents are synthesized, how they facilitate rapid assays, and a recently developed binary reagent system that enhances assay performance in both low-resource settings and centralized laboratories.
Stimuli-responsive Reagent Systems for Point-of-Care Diagnostics
University of Washington
Tuesday, April 16, 2013
Duke University, Schiciano B | 3:15pm