Stimuli-responsive Reagent Systems for Point-of-Care Diagnostics

James Lai
University of Washington
Tuesday, April 16, 2013
Duke University, Schiciano B | 3:15pm

Clinical diagnostic assays for detecting biomarkers can potentially help in the identification and epidemiological tracking of many diseases, as well as in their treatment monitoring.  Point-of-care immunoassays such as lateral flow tests are cost-effective analytical tests, which can be performed in low-resource settings with untrained users to provide more immediate diagnosis.  However, the major drawback is poor sensitivity.  To address the need, we have developed a family of stimuli-responsive reagents that can enable easy-to-use rapid diagnostic assays to achieve ELISA level sensitivity.  The new reagent systems can isolate full or half-sandwich immunocomplexes from human plasma and directly interface with different diagnostic devices such as microfluidic cards and non-instrumented flow-through devices. Additionally, the rapid assay system is scalable to larger specimen volumes (≥ 10x), which provides opportunities to concentrate dilute biomarkers, thus improving detection ranges and expanding diagnostic options in point-of-care immunoassays.  In this presentation, I will describe how the reagents are synthesized, how they facilitate rapid assays, and a recently developed binary reagent system that enhances assay performance in both low-resource settings and centralized laboratories.